Journal: Medical Sciences
Article Title: Effectiveness of a Two-Step Testing Algorithm for Reliable and Cost-Effective Detection of Clostridium difficile Infection in a Tertiary Care Hospital in Saudi Arabia
doi: 10.3390/medsci7010006
Figure Lengend Snippet: The two-step algorithm for C. difficile testing using Quik Chek Complete dual-antigen enzyme immunoassay (EIA) and Xpert C. difficile PCR. Stool samples were initially screened using the Quik Chek Complete EIA test. Samples which yielded discrepant results (i.e., positive for antigen and negative for toxin) were tested by PCR. GDH-Glutamate Dehydrogenase.
Article Snippet: During phase two of the two-step algorithm, a total of 3518 stool specimens were initially tested using the C. diff Quik Chek Complete ® dual-antigen EIA test (D-EIA; TechLab, Blacksburg VA, U.S.A.), and the discordant results were confirmed by Xpert C. difficile PCR assay following the manufacturer’s instructions.
Techniques: Enzyme-linked Immunosorbent Assay